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Locations (A) and sequences (B) of the primers and probe in the <t>BKV</t> genome. Broad lines indicate open reading frames. Broad purple lines indicate the early region which is predominantly expressed before viral DNA replication to encodes the large T-antigen (T-ag) and small t-antigen (t-ag); broad blue lines indicate the late region which is expressed late in the viral life cycle to encodes the agnoprotein and capsid <t>proteins</t> <t>VP1,</t> VP2, and VP3.
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Locations (A) and sequences (B) of the primers and probe in the <t>BKV</t> genome. Broad lines indicate open reading frames. Broad purple lines indicate the early region which is predominantly expressed before viral DNA replication to encodes the large T-antigen (T-ag) and small t-antigen (t-ag); broad blue lines indicate the late region which is expressed late in the viral life cycle to encodes the agnoprotein and capsid <t>proteins</t> <t>VP1,</t> VP2, and VP3.
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Locations (A) and sequences (B) of the primers and probe in the BKV genome. Broad lines indicate open reading frames. Broad purple lines indicate the early region which is predominantly expressed before viral DNA replication to encodes the large T-antigen (T-ag) and small t-antigen (t-ag); broad blue lines indicate the late region which is expressed late in the viral life cycle to encodes the agnoprotein and capsid proteins VP1, VP2, and VP3.

Journal: PeerJ

Article Title: Rapid point-of-care detection of BK virus in urine by an HFman probe-based loop-mediated isothermal amplification assay and a finger-driven microfluidic chip

doi: 10.7717/peerj.14943

Figure Lengend Snippet: Locations (A) and sequences (B) of the primers and probe in the BKV genome. Broad lines indicate open reading frames. Broad purple lines indicate the early region which is predominantly expressed before viral DNA replication to encodes the large T-antigen (T-ag) and small t-antigen (t-ag); broad blue lines indicate the late region which is expressed late in the viral life cycle to encodes the agnoprotein and capsid proteins VP1, VP2, and VP3.

Article Snippet: PUC-57 plasmids containing the VP1 gene (GenBank accession no: AB298947.1 , 1610–2363 nt) of BKV were synthesized by Sangon Biotech (Shanghai, China) as standards.

Techniques: